2E0G
DnaA N-terminal domain
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | 0.8mM DnaA N-terminal domain U-15N, 13C, 50mM phospate buffer (pH6.5), 20mM EDTA, 40mM KCl, 2mM DTT, 10% sucrose; 90% H2O, 10% D2O | 90% H2O/10% D2O | 6.5 | AMBIENT | 298 | ||
| 2 | 3D_13C-separated_NOESY | 0.8mM DnaA N-terminal domain U-13C, 50mM phospate buffer (pH6.5), 20mM EDTA, 40mM KCl, 2mM DTT, 10% sucrose; 100% D2O | 100% D2O | 6.5 | AMBIENT | 298 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | CYANA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 25 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | CYANA | 2.1 | P.GUNTERT ET AL. |
| 2 | structure solution | CYANA | 2.1 | |
