7QRM

Cryo-EM structure of catalytically active Spinacia oleracea cytochrome b6f in complex with endogenous plastoquinones at 2.7 A resolution


ELECTRON MICROSCOPY
Sample
Cytochrome b6f complex
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles97597
Reported Resolution (Å)2.7
Resolution MethodFSC 0.143 CUT-OFF
Other DetailsBatchsize snrfactor tuned to 300
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1
Refinement Space
Refinement ProtocolAB INITIO MODEL
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeGATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å**2)40
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelTFS KRIOS
Minimum Defocus (nm)900
Maximum Defocus (nm)2100
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification105000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
PARTICLE SELECTIONcryoSPARCv3.3.1
CTF CORRECTIONcryoSPARCv3.3.1
INITIAL EULER ASSIGNMENTcryoSPARCv3.3.1
FINAL EULER ASSIGNMENTcryoSPARCv3.3.1
CLASSIFICATIONcryoSPARCv3.3.1
RECONSTRUCTIONcryoSPARCv3.3.1
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION2 iteration in global CTF refinement, with an anisotropic mag. fitting177412given number of particles is after template picker and 2D cleaning