6VEJ

TriABC transporter from Pseudomonas aeruginosa


ELECTRON MICROSCOPY
Sample
TriABC
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification Instrument
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles35220
Reported Resolution (Å)4.5
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Map-Model Fitting and Refinement
Id1 (3NE5, 3LNN, 2V4D)
Refinement SpaceREAL
Refinement ProtocolAB INITIO MODEL
Refinement Target
Overall B Value
Fitting Procedure
DetailsSubunits A-C were modeled by I-TASSER using CusA (3NE5) and then fitted into the EM density and refined with Phenix and model rebuilt using Coot. The ...Subunits A-C were modeled by I-TASSER using CusA (3NE5) and then fitted into the EM density and refined with Phenix and model rebuilt using Coot. The N-terminal regions of subunits P-R were modeled by I-TASSER based on MP domain of 3LNN while C-terminal regions of subunits P-R were modelled based on MP domain of 2V4D and similarly refined and model rebuilt.
Data Acquisition
Detector TypeFEI FALCON II (4k x 4k)
Electron Dose (electrons/Å**2)50
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)1600
Maximum Defocus (nm)3000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification75000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
PARTICLE SELECTIONEMAN2
IMAGE ACQUISITIONEPU
CTF CORRECTIONCTFFIND4
INITIAL EULER ASSIGNMENTRELION1.3
FINAL EULER ASSIGNMENTRELION1.3
RECONSTRUCTIONRELION1.3
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION35530