5A1X

The structure of the COPI coat linkage III


ELECTRON MICROSCOPY
Sample
COPI COAT LINKAGE III ON THE MEMBRANE
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentHOMEMADE PLUNGER
Cryogen NameETHANE
Sample Vitrification DetailsLIQUID ETHANE
3D Reconstruction
Reconstruction MethodTOMOGRAPHY
Number of Particles421
Reported Resolution (Å)23
Resolution Method
Other DetailsHOMOLOGY MODELS WERE FITTED INTO THE EM MAP USING THE MDFF (MOLECULAR DYNAMICS FLEXIBLE FITTING) SOFTWARE. DUE TO LOW RESOLUTION OF THE EM MAP USED FO ...HOMOLOGY MODELS WERE FITTED INTO THE EM MAP USING THE MDFF (MOLECULAR DYNAMICS FLEXIBLE FITTING) SOFTWARE. DUE TO LOW RESOLUTION OF THE EM MAP USED FOR FITTING, WE DEPOSIT ONLY THE BACKBONE ATOMS. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2988. (DEPOSITION ID: 13342).
Refinement Type
Symmetry TypePOINT
Point SymmetryC2
Map-Model Fitting and Refinement
Id1
Refinement SpaceREAL
Refinement ProtocolOTHER
Refinement TargetCross-correlation coefficient
Overall B Value
Fitting Procedure
DetailsMETHOD--CROSS-CORRELATION REFINEMENT PROTOCOL--HOMOLOGY-MODEL
Data Acquisition
Detector TypeGATAN MULTISCAN
Electron Dose (electrons/Å**2)45
Imaging Experiment1
Date of Experiment2014-02-07
Temperature (Kelvin)90
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)1500
Maximum Defocus (nm)4000
Minimum Tilt Angle (degrees)-45
Maximum Tilt Angle (degrees)60
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification42000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)200
Imaging Details
EM Software
TaskSoftware PackageVersion
RECONSTRUCTIONAV3
RECONSTRUCTIONTOM Toolbox
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING OF INDIVIDUAL TILTS