4BOG
The structure and super-organization of acetylcholine receptor-rapsyn complexes
ELECTRON MICROSCOPY
| Sample |
|---|
| ACETYLCHOLINE RECEPTOR- RAPSYN COMPLEX |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | HOMEMADE PLUNGER |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | ETHANE |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | TOMOGRAPHY |
| Number of Particles | 3564 |
| Reported Resolution (Å) | 50 |
| Resolution Method | |
| Other Details | SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2376. (DEPOSITION ID: 11658). |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C1 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (2BG9) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | OTHER | ||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--LOCAL CORRELATION REFINEMENT PROTOCOL--ELECTRON MICROSCOPY | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | GATAN ULTRASCAN 4000 (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 50 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2008-07-25 |
| Temperature (Kelvin) | 85 |
| Microscope Model | FEI TECNAI F30 |
| Minimum Defocus (nm) | 3000 |
| Maximum Defocus (nm) | 6000 |
| Minimum Tilt Angle (degrees) | -70 |
| Maximum Tilt Angle (degrees) | 70 |
| Nominal CS | 2 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 69000 |
| Calibrated Magnification | 80213 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | UCSF Chimera | |
| RECONSTRUCTION | IMOD | |
| RECONSTRUCTION | PRIISM/IVE | |
| RECONSTRUCTION | TOMO3D | |
| RECONSTRUCTION | Xmipp | |
