4A9G

Symmetrized cryo-EM reconstruction of E. coli DegQ 24-mer in complex with beta-casein


ELECTRON MICROSCOPY
Sample
DEGQ 24-MER BOUND TO BETA- CASEIN
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentHOMEMADE PLUNGER
Cryogen NameETHANE
Sample Vitrification DetailsLIQUID ETHANE
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles9848
Reported Resolution (Å)7.5
Resolution Method
Other DetailsBETA-CASEIN WERE NOT FITTED AS BETA-CASEIN IS AN INTRISICALLY DISORDERED PROTEIN. HOWEVER BETA-CASEIN IS PRESENT IN THE DEGQ 24-MER COMPLEX. SUBMISSIO ...BETA-CASEIN WERE NOT FITTED AS BETA-CASEIN IS AN INTRISICALLY DISORDERED PROTEIN. HOWEVER BETA-CASEIN IS PRESENT IN THE DEGQ 24-MER COMPLEX. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1984. (DEPOSITION ID: 10375).
Refinement Type
Symmetry TypePOINT
Point SymmetryO
Map-Model Fitting and Refinement
Id1 (3STJ)
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement TargetCross-correlation coefficient
Overall B Value
Fitting Procedure
DetailsMETHOD--FLEXIBLE FITTING REFINEMENT PROTOCOL--X-RAY
Data Acquisition
Detector TypeKODAK SO-163 FILM
Electron Dose (electrons/Å**2)15
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)91
Microscope ModelFEI TECNAI F20
Minimum Defocus (nm)1000
Maximum Defocus (nm)3000
Minimum Tilt Angle (degrees)-0.5
Maximum Tilt Angle (degrees)
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification50000
Calibrated Magnification50000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)200
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGFlex-EM
MODEL FITTINGMODELLER
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONIMAGIC
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
FULL CTF CORRECTION