3IYG

Ca model of bovine TRiC/CCT derived from a 4.0 Angstrom cryo-EM map


ELECTRON MICROSCOPY
Sample
bovine TRiC (also called CCT)
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification Instrument
Cryogen NameETHANE
Sample Vitrification Detailsvitrification using ethane as cryogen
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles101000
Reported Resolution (Å)4
Resolution MethodFSC 0.5 CUT-OFF
Other DetailsA recently developed 2-D fast rotational matching (FRM2D) algorithm for image alignment, available in EMAN 1.8, was adopted in the refinement steps ( ...A recently developed 2-D fast rotational matching (FRM2D) algorithm for image alignment, available in EMAN 1.8, was adopted in the refinement steps ( Details about the particle: A recently developed 2-D fast rotational matching (FRM2D) algorithm for image alignment, available in EMAN 1.8, was adopted in the refinement steps )
Refinement Type
Symmetry TypePOINT
Point SymmetryC2
Map-Model Fitting and Refinement
Id1
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--Local refinement, Flexible fitting REFINEMENT PROTOCOL--Local refinement, Flexible fitting
Data Acquisition
Detector TypeKODAK SO-163 FILM
Electron Dose (electrons/Å**2)18
Imaging Experiment1
Date of Experiment2007-08-01
Temperature (Kelvin)101
Microscope ModelJEOL 3200FSC
Minimum Defocus (nm)1000
Maximum Defocus (nm)3000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS4.1
Imaging ModeBRIGHT FIELD
Specimen Holder ModelJEOL 3200FSC CRYOHOLDER
Nominal Magnification50000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGCoot
MODEL FITTINGMODELLER
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONEMAN
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
each micrograph