2ZLE

Cryo-EM structure of DegP12/OMP

ELECTRON MICROSCOPY

Sample
DegP24mer with bound Omp

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	FEI VITROBOT MARK I
Cryogen Name	ETHANE
Sample Vitrification Details	Embedded in vitreous ice using C-flat holey carbon grids (CF-2/2-4C-100, Protochip) and a Vitrobot (FEI) at 20 temperature and 100% relative humidity

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	6285
Reported Resolution (Å)	28
Resolution Method
Other Details	This structure is docking of four DegP trimers (PDB ID 1KY9) and an OmpC monomer (PDB ID 2J1N) into an EM map by hand at 28A resolution. At this low ...	This structure is docking of four DegP trimers (PDB ID 1KY9) and an OmpC monomer (PDB ID 2J1N) into an EM map by hand at 28A resolution. At this low resolution, no exact fitting can be done.
Refinement Type
Symmetry Type	POINT
Point Symmetry	C1

Data Acquisition

Imaging Experiment	1
Date of Experiment
Temperature (Kelvin)
Microscope Model	FEI TECNAI F20
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging Mode	OTHER
Specimen Holder Model
Nominal Magnification	68100
Calibrated Magnification
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	200
Imaging Details

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	phase flipping

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.