NMR Experiment |
---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
---|
1 | 2D 1H-15N HSQC | 0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
2 | 2D 1H-13C HSQC aliphatic | 0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
3 | 2D 1H-13C HSQC aromatic | 0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
4 | 3D HNCA | 0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
5 | 3D HN(CO)CA | 0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
6 | 3D HCCH-TOCSY | 0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
7 | 3D 13C,15N-filtered NOESY | 0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
8 | 4D 13C, 15N-edited NOESY | 0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
9 | 2D 1H-15N HSQC | 0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
10 | 2D 1H-13C HSQC aliphatic | 0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
11 | 2D 1H-13C HSQC aromatic | 0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
12 | 3D HNCA | 0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
13 | 3D HN(CO)CA | 0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
14 | 3D HCCH-TOCSY | 0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
15 | 3D 13C,15N-filtered NOESY | 0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |
16 | 4D 13C, 15N-edited NOESY | 0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12 | 95% H2O/5% D2O | 10 | 6.9 | ambient | 301 | |