2EAN

Solution structure of the N-terminal SAM-domain of human KIAA0902 protein (connector enhancer of kinase suppressor of ras 2)

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_15N-separated_NOESY	1.27mM SAM DOMAIN, 20mM d-TRIS-HCL, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O	90% H2O/10% D2O	120mM	7.0	AMBIENT	296
2	3D_13C-separated_NOESY	1.27mM SAM DOMAIN, 20mM d-TRIS-HCL, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O	90% H2O/10% D2O	120mM	7.0	AMBIENT	296

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	800

NMR Refinement
Method	Details	Software
torsion angle dynamics		CYANA

NMR Ensemble Information
Conformer Selection Criteria	structures with the least restraint violations
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (fewest violations)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	CYANA	2.2.2	P.GUNTERT ET AL.
2	structure solution	CYANA	2.2.2	P.GUNTERT ET AL.
3	collection	XwinNMR	3.5	BRUKER
4	processing	NMRPipe	20060702	FRANK DELAGLIO
5	data analysis	NMRView	5.0.4	BRUCE A. JOHNSON
6	data analysis	KUJIRA	0.9820	NAOHIRO KOBAYASHI

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.