1YSE

Solution structure of the MAR-binding domain of SATB1


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D NOESY1mM SATB1 MAR-binding domain, 50mM phosphate buffer NA; 95% H2O, 10% D2O95% H2O/5% D2O50mM phosphate buffer5.51 atm308
22D TOCSY1mM SATB1 MAR-binding domain, 50mM phosphate buffer NA; 95% H2O, 10% D2O95% H2O/5% D2O50mM phosphate buffer5.51 atm308
33D_13C-separated_NOESY1mM SATB1 MAR-binding domain, 50mM phosphate buffer NA; 95% H2O, 10% D2O95% H2O/5% D2O50mM phosphate buffer5.51 atm308
43D_15N-separated_NOESY1mM SATB1 MAR-binding domain, 50mM phosphate buffer NA; 95% H2O, 10% D2O95% H2O/5% D2O50mM phosphate buffer5.51 atm308
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDMX750
2BrukerDMX500
NMR Refinement
MethodDetailsSoftware
simulated annealingthe structures are based on a total of 1184 restraints: 989 NOE-derived distance constraints, 109 dihedral angle restraints,and 86 distance restraints from hydrogen bonds.XwinNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number50
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1processingXwinNMR3.5Bruker
2data analysisFelix2000Accelrys
3structure solutionCNS1.1Brunger
4refinementCNS1.1Brunger