1R5S
Connexin 43 Carboxyl Terminal Domain
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | 1.5mM Cx43CT U-15N,13C, PBS | 95% H2O/5% D2O | PBS | 5.8 | ambient | 280 | |
| 2 | 3D_15N-separated_NOESY | 1.5mM Cx43CT U-15N,13C, PBS | 95% H2O/5% D2O | PBS | 5.8 | ambient | 280 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics, simulated annealing | CNS | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 10 |
| Representative Model | 1 (lowest energy) |
| Additional NMR Experimental Information | |
|---|---|
| Details | This structure was determined using standard 3D homonuclear techniques |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | CNS | 1.1 | Brunger, et al. |
| 2 | processing | NMRPipe | 7.2 | Delaglio, et al. |
| 3 | refinement | CNS | 1.1 | Brunger, et al. |
