1P97

NMR structure of the C-terminal PAS domain of HIF2a

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	0.9mM HIF2a (240-350) U-15N,13C; 50 mM Tris buffer, 15 mM NaCl, 5 mM DTT (pH 7.3); 90% H2O, 10% D2O	90% H2O/10% D2O	15 mM - 75 mM	7.3	ambient	303
2	3D_15N-separated_NOESY	0.9mM HIF2a (240-350) U-15N,13C; 50 mM Tris buffer, 15 mM NaCl, 5 mM DTT (pH 7.3); 90% H2O, 10% D2O	90% H2O/10% D2O	15 mM - 75 mM	7.3	ambient	303
3	2D NOESY	0.9mM HIF2a (240-350) U-15N,13C; 50 mM Tris buffer, 15 mM NaCl, 5 mM DTT (pH 7.3); 90% H2O, 10% D2O	90% H2O/10% D2O	15 mM - 75 mM	7.3	ambient	303

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	500
2	Varian	INOVA	600

NMR Refinement
Method	Details	Software
simulated annealing		CNS

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	1000
Conformers Submitted Total Number	20

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	CNS	1.1	Brunger

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.