1HN3
SOLUTION STRUCTURE OF THE N-TERMINAL 37 AMINO ACIDS OF THE MOUSE ARF TUMOR SUPPRESSOR PROTEIN
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_15N-separated_NOESY | 1mM mArfN37 15N or 15N/13C; 10mM potassium phosphate, pH 5.0; 50mM NaCl | 5% D2O; 30% TFE (v/v) in water | 60 | 5.0 | ambient | 313 | |
| 2 | 3D_13C-separated_NOESY | 1mM mArfN37 15N or 15N/13C; 10mM potassium phosphate, pH 5.0; 50mM NaCl | 5% D2O; 30% TFE (v/v) in water | 60 | 5.0 | ambient | 313 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| CNS was used for structure calculations | VNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 11 (extended conformation) |
| Additional NMR Experimental Information | |
|---|---|
| Details | Backbone dihedral angle restraints were obtained using the program TALOS developed by Cornilescu, G., Delaglio, F. and Bax, A. |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | VNMR | 6.1 | varian |
| 2 | processing | NMRPipe | 1.7 | Delaglio, F. |
| 3 | data analysis | Felix | 98 | MSI |
| 4 | structure solution | CNS | 1.0 | Brunger, A.T. |
| 5 | refinement | CNS | 1.0 | Brunger, A.T. |
