5NDF

Small-molecule inhibition of ppGalNAc-Ts selectively reduces mucin-type O-glycosylation

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Ricin_B_lectin	e5ndfA2	A: beta duplicates or obligate multimers	X: beta-Trefoil (From Topology)	H: beta-Trefoil (From Topology)	T: beta-Trefoil	F: Ricin_B_lectin	ECOD (1.6)
A	Glycos_transf_2	e5ndfA1	A: a/b three-layered sandwiches	X: Nucleotide-diphospho-sugar transferases (From Topology)	H: Nucleotide-diphospho-sugar transferases (From Topology)	T: Nucleotide-diphospho-sugar transferases	F: Glycos_transf_2	ECOD (1.6)
B	Ricin_B_lectin	e5ndfB2	A: beta duplicates or obligate multimers	X: beta-Trefoil (From Topology)	H: beta-Trefoil (From Topology)	T: beta-Trefoil	F: Ricin_B_lectin	ECOD (1.6)
B	Glycos_transf_2	e5ndfB1	A: a/b three-layered sandwiches	X: Nucleotide-diphospho-sugar transferases (From Topology)	H: Nucleotide-diphospho-sugar transferases (From Topology)	T: Nucleotide-diphospho-sugar transferases	F: Glycos_transf_2	ECOD (1.6)
C	Ricin_B_lectin	e5ndfC1	A: beta duplicates or obligate multimers	X: beta-Trefoil (From Topology)	H: beta-Trefoil (From Topology)	T: beta-Trefoil	F: Ricin_B_lectin	ECOD (1.6)
C	Glycos_transf_2	e5ndfC2	A: a/b three-layered sandwiches	X: Nucleotide-diphospho-sugar transferases (From Topology)	H: Nucleotide-diphospho-sugar transferases (From Topology)	T: Nucleotide-diphospho-sugar transferases	F: Glycos_transf_2	ECOD (1.6)
D	Ricin_B_lectin	e5ndfD1	A: beta duplicates or obligate multimers	X: beta-Trefoil (From Topology)	H: beta-Trefoil (From Topology)	T: beta-Trefoil	F: Ricin_B_lectin	ECOD (1.6)
D	Glycos_transf_2	e5ndfD2	A: a/b three-layered sandwiches	X: Nucleotide-diphospho-sugar transferases (From Topology)	H: Nucleotide-diphospho-sugar transferases (From Topology)	T: Nucleotide-diphospho-sugar transferases	F: Glycos_transf_2	ECOD (1.6)
F	Ricin_B_lectin	e5ndfF1	A: beta duplicates or obligate multimers	X: beta-Trefoil (From Topology)	H: beta-Trefoil (From Topology)	T: beta-Trefoil	F: Ricin_B_lectin	ECOD (1.6)
F	Glycos_transf_2	e5ndfF2	A: a/b three-layered sandwiches	X: Nucleotide-diphospho-sugar transferases (From Topology)	H: Nucleotide-diphospho-sugar transferases (From Topology)	T: Nucleotide-diphospho-sugar transferases	F: Glycos_transf_2	ECOD (1.6)
E	Ricin_B_lectin	e5ndfE1	A: beta duplicates or obligate multimers	X: beta-Trefoil (From Topology)	H: beta-Trefoil (From Topology)	T: beta-Trefoil	F: Ricin_B_lectin	ECOD (1.6)
E	Glycos_transf_2	e5ndfE2	A: a/b three-layered sandwiches	X: Nucleotide-diphospho-sugar transferases (From Topology)	H: Nucleotide-diphospho-sugar transferases (From Topology)	T: Nucleotide-diphospho-sugar transferases	F: Glycos_transf_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.550.10	Alpha Beta	Alpha-Beta Complex	Spore Coat Polysaccharide Biosynthesis Protein SpsA	Chain A	CATH (4.3.0)
B	3.90.550.10	Alpha Beta	Alpha-Beta Complex	Spore Coat Polysaccharide Biosynthesis Protein SpsA	Chain A	CATH (4.3.0)
C	3.90.550.10	Alpha Beta	Alpha-Beta Complex	Spore Coat Polysaccharide Biosynthesis Protein SpsA	Chain A	CATH (4.3.0)
D	3.90.550.10	Alpha Beta	Alpha-Beta Complex	Spore Coat Polysaccharide Biosynthesis Protein SpsA	Chain A	CATH (4.3.0)
F	3.90.550.10	Alpha Beta	Alpha-Beta Complex	Spore Coat Polysaccharide Biosynthesis Protein SpsA	Chain A	CATH (4.3.0)
E	3.90.550.10	Alpha Beta	Alpha-Beta Complex	Spore Coat Polysaccharide Biosynthesis Protein SpsA	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF00535	Glycosyl transferase family 2 (Glycos_transf_2)	Glycosyl transferase family 2	-	Family
A, B, C, D, E A, B, C, D, E, F	PF00652	Ricin-type beta-trefoil lectin domain (Ricin_B_lectin)	Ricin-type beta-trefoil lectin domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	Polypeptide N-acetylgalactosaminyltransferase 2	binding carbohydrate binding cation binding manganese ion binding transition metal ion binding ion binding metal ion binding small molecule binding UDP-glycosyltransferase activity polypeptide N-acetylgalactosaminyltransferase activity catalytic activity, acting on a protein glycosyltransferase activity transferase activity acetylgalactosaminyltransferase activity binding carbohydrate binding cation binding manganese ion binding transition metal ion binding ion binding metal ion binding small molecule binding UDP-glycosyltransferase activity polypeptide N-acetylgalactosaminyltransferase activity catalytic activity, acting on a protein glycosyltransferase activity transferase activity acetylgalactosaminyltransferase activity hexosyltransferase activity catalytic activity	cellular biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process organic substance metabolic process organic substance biosynthetic process peptidyl-amino acid modification protein metabolic process peptidyl-threonine modification protein O-linked glycosylation via threonine protein modification process carbohydrate derivative biosynthetic process organonitrogen compound metabolic process cellular metabolic process glycoprotein biosynthetic process cellular biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process organic substance metabolic process organic substance biosynthetic process peptidyl-amino acid modification protein metabolic process peptidyl-threonine modification protein O-linked glycosylation via threonine protein modification process carbohydrate derivative biosynthetic process organonitrogen compound metabolic process cellular metabolic process glycoprotein biosynthetic process primary metabolic process glycoprotein metabolic process protein glycosylation macromolecule biosynthetic process biosynthetic process glycosylation macromolecule modification macromolecule glycosylation protein O-linked glycosylation metabolic process cellular process macromolecule metabolic process gene expression protein maturation peptidyl-serine modification protein O-linked glycosylation via serine O-glycan processing	membrane-bounded organelle intracellular anatomical structure Golgi apparatus subcompartment Golgi apparatus organelle subcompartment cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle Golgi stack cytoplasm membrane extracellular region membrane-bounded organelle intracellular anatomical structure Golgi apparatus subcompartment Golgi apparatus organelle subcompartment cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle Golgi stack cytoplasm membrane extracellular region Golgi membrane bounding membrane of organelle organelle membrane Golgi cisterna membrane Golgi cisterna perinuclear region of cytoplasm endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum endoplasmic reticulum membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR045885	N-acetylgalactosaminyltransferase	Domain
A, B, C, D, E A, B, C, D, E, F	IPR035992	Ricin B-like lectins	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR029044	Nucleotide-diphospho-sugar transferases	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR001173	Glycosyltransferase 2-like	Domain
A, B, C, D, E A, B, C, D, E, F	IPR000772	Ricin B, lectin domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D, E A, B, C, D, E, F	Pharos: Q10471	metabolic syndrome X pancreatic ductal adenocarcinoma pilocytic astrocytoma astrocytic tumor breast carcinoma diabetes mellitus lung adenocarcinoma malignant ependymoma osteosarcoma pancreatic intraductal papillary-mucinous neoplasm plasma cell myeloma atypical teratoid rhabdoid tumor congenital disorder of glycosylation gastric carcinoma lung cancer metabolic syndrome X pancreatic ductal adenocarcinoma pilocytic astrocytoma astrocytic tumor breast carcinoma diabetes mellitus lung adenocarcinoma malignant ependymoma osteosarcoma pancreatic intraductal papillary-mucinous neoplasm plasma cell myeloma atypical teratoid rhabdoid tumor congenital disorder of glycosylation gastric carcinoma lung cancer malignant glioma non-small cell lung carcinoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia ulcerative colitis breast cancer Crohn disease glioblastoma lymphoplasmacytic lymphoma malignant mesothelioma oligodendroglioma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.