2AKI

Normal mode-based flexible fitted coordinates of a translocating SecYEG protein-conducting channel into the cryo-EM map of a SecYEG-nascent chain-70S ribosome complex from E. coli


ELECTRON MICROSCOPY
Sample
protein-conducting channel
Sample Components
protein translocase activity
protein translocase activity
protein translocase activity
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentHOMEMADE PLUNGER
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles53325
Reported Resolution (Å)14.9
Resolution MethodFSC 0.5 CUT-OFF
Other DetailsThe resolution is based on FSC at 0.5 cut-off
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement Targetcorrelation coefficient, R-factor
Overall B Value
Fitting Procedure
DetailsMETHOD--normal mode-based flexible fitting REFINEMENT PROTOCOL--normal mode-based flexible fitting, real space refinement
Data Acquisition
Detector TypeKODAK SO-163 FILM
Electron Dose (electrons/Å**2)11
Imaging Experiment1
Date of Experiment2004-03-09
Temperature (Kelvin)93
Microscope ModelFEI TECNAI F30
Minimum Defocus (nm)1500
Maximum Defocus (nm)4300
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.26
Imaging ModeBRIGHT FIELD
Specimen Holder ModelOTHER
Nominal Magnification39000
Calibrated Magnification39000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGRSR2000
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
defocus groups