1ZO3

The P-site and P/E-site tRNA structures fitted to P/I site codon.


ELECTRON MICROSCOPY
Sample
70S INITIATION COMPLEX
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK I
Cryogen NameETHANE
Sample Vitrification DetailsHOLEY CARBON GRID AT 20C, FLASH FROZEN INTO LIQUID ETHAN
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles20283
Reported Resolution (Å)13.8
Resolution MethodFSC 0.5 CUT-OFF
Other Detailsresolution 13.8 ANGSTROMS (FSC=0.5) and 8.6 ANGSTROMS (3sigma)
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement TargetBEST FIT USING RSREF
Overall B Value
Fitting Procedure
DetailsREFINEMENT PROTOCOL--RIGID BODY FIT OF TRNA DETAILS--the tRNA coordinate files were fitted to the E. coli translation initiation complex map (EMBL-EM ...REFINEMENT PROTOCOL--RIGID BODY FIT OF TRNA DETAILS--the tRNA coordinate files were fitted to the E. coli translation initiation complex map (EMBL-EMD 3525) in the following way- first the cross-correlation of the respective 30S subunit maps were maximized and the resulting transformation matrix was computed. second the coordinates of the tRNA models were transformed according to the matrix previously computed.
Data Acquisition
Detector TypeKODAK SO-163 FILM
Electron Dose (electrons/Å**2)
Imaging Experiment1
Date of Experiment2004-01-01
Temperature (Kelvin)100
Microscope ModelFEI TECNAI F20
Minimum Defocus (nm)-930
Maximum Defocus (nm)-3930
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification39000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)200
Imaging DetailsLOW DOSE MODE
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
DEFOCUS GROUPS 0.93-3.93 UM