Download MendeleyCopper inactivates DcsB by oxidation of the Cys86 to cysteine sulfinic aicd
Oda, K., Komaguchi, K., Matoba, Y.To be published.
8IUV
Crystal structure of Manganese-free N(omega)-hydroxy-L-arginine hydrolase with oxidized Cys86
- PDB DOI: https://doi.org/10.2210/pdb8IUV/pdb
- Classification: HYDROLASE
- Organism(s): Streptomyces lavendulae
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2023-03-25 Released: 2024-03-27
- Funding Organization(s): Not funded
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.201
- R-Value Work: 0.158
- R-Value Observed: 0.160
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| N(omega)-hydroxy-L-arginine amidinohydrolase | 281 | Streptomyces lavendulae | Mutation(s): 0 Gene Names: dcsB EC: 3.5.3.25 |  | |
UniProt | |||||
Find proteins for D2Z025 (Streptomyces lavendulae) Explore D2Z025 Go to UniProtKB: D2Z025 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | D2Z025 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MN (Subject of Investigation/LOI) Query on MN | C [auth A] | MANGANESE (II) ION Mn WAEMQWOKJMHJLA-UHFFFAOYSA-N |  | ||
| MG (Subject of Investigation/LOI) Query on MG | D [auth A], E [auth B], F [auth B], G [auth B] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| CSO Query on CSO | A, B | L-PEPTIDE LINKING | C3 H7 N O3 S |  | CYS |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.201
- R-Value Work: 0.158
- R-Value Observed: 0.160
- Space Group: P 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 45.876 | α = 85.39 |
| b = 46.683 | β = 86.78 |
| c = 58.222 | γ = 70.54 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| Aimless | data scaling |
| XDS | data reduction |
| PHASES | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-03-27 Deposition Author(s): Oda, K., Matoba, Y.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Not funded | -- |
Revision History (Full details and data files)
- Version 1.0: 2024-03-27
Type: Initial release
