7EHA

Crystal structure of the flagellar hook cap from Salmonella enterica serovar Typhimurium

PDB DOI: https://doi.org/10.2210/pdb7EHA/pdb
Entry: 7EHA supersedes: 6IEE

Classification: MOTOR PROTEIN
Organism(s): Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2021-03-29 Released: 2021-11-24
Deposition Author(s): Matsunami, H., Yoon, Y.-H., Imada, K., Namba, K., Samatey, F.A.
Funding Organization(s): Japan Society for the Promotion of Science (JSPS)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 3.30 Å
R-Value Free: 0.284
R-Value Work: 0.244
R-Value Observed: 0.246

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Structure of the bacterial flagellar hook cap provides insights into a hook assembly mechanism

Matsunami, H., Yoon, Y.H., Imada, K., Namba, K., Samatey, F.A.

(2021) Commun Biol 4: 1291

DOI: https://doi.org/10.1038/s42003-021-02796-6

Macromolecule Content

Total Structure Weight: 120.01 kDa
Atom Count: 5,988
Modelled Residue Count: 824
Deposited Residue Count: 1,160
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Basal-body rod modification protein FlgD	A, B, C, D, E	232	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	Mutation(s): 0 Gene Names: flgD, fla FIV, flaV, STM1176
UniProt
Find proteins for P0A1I9 (Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)) Explore P0A1I9 Go to UniProtKB: P0A1I9
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P0A1I9
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 3.30 Å
R-Value Free: 0.284
R-Value Work: 0.244
R-Value Observed: 0.246
Space Group: P 3₂ 2 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 141.283	α = 90
b = 141.283	β = 90
c = 153.487	γ = 120

Software Package:

Software Name	Purpose
BSS	data collection
MOSFLM	data reduction
SCALA	data scaling
SHARP	phasing
PHENIX	refinement

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2021-11-24

Deposition Author(s):

This entry supersedes: 6IEE

Funding Organization	Location	Grant Number
Japan Society for the Promotion of Science (JSPS)	Japan	--

Revision History (Full details and data files)

Version 1.0: 2021-11-24
Type: Initial release

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.