6JPD

Mouse receptor-interacting protein kinase 3 (RIP3) amyloid structure by solid-state NMR


Experimental Data Snapshot

  • Method: SOLID-STATE NMR
  • Conformers Calculated: 200 
  • Conformers Submitted: 10 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

The amyloid structure of mouse RIPK3 (receptor interacting protein kinase 3) in cell necroptosis.

Wu, X.L.Hu, H.Dong, X.Q.Zhang, J.Wang, J.Schwieters, C.D.Liu, J.Wu, G.X.Li, B.Lin, J.Y.Wang, H.Y.Lu, J.X.

(2021) Nat Commun 12: 1627-1627

  • DOI: https://doi.org/10.1038/s41467-021-21881-2
  • Primary Citation of Related Structures:  
    6JPD

  • PubMed Abstract: 

    RIPK3 amyloid complex plays crucial roles during TNF-induced necroptosis and in response to immune defense in both human and mouse. Here, we have structurally characterized mouse RIPK3 homogeneous self-assembly using solid-state NMR, revealing a well-ordered N-shaped amyloid core structure featured with 3 parallel in-register β-sheets. This structure differs from previously published human RIPK1/RIPK3 hetero-amyloid complex structure, which adopted a serpentine fold. Functional studies indicate both RIPK1-RIPK3 binding and RIPK3 amyloid formation are essential but not sufficient for TNF-induced necroptosis. The structural integrity of RIPK3 fibril with three β-strands is necessary for signaling. Molecular dynamics simulations with a mouse RIPK1/RIPK3 model indicate that the hetero-amyloid is less stable when adopting the RIPK3 fibril conformation, suggesting a structural transformation of RIPK3 from RIPK1-RIPK3 binding to RIPK3 amyloid formation. This structural transformation would provide the missing link connecting RIPK1-RIPK3 binding to RIPK3 homo-oligomer formation in the signal transduction.


  • Organizational Affiliation

    School of Life Science and Technology, ShanghaiTech University, Shanghai, PR China.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Receptor-interacting serine/threonine-protein kinase 3
A, B, C, D, E
84Mus musculusMutation(s): 0 
Gene Names: Ripk3Rip3
EC: 2.7.11.1
UniProt & NIH Common Fund Data Resources
Find proteins for Q9QZL0 (Mus musculus)
Explore Q9QZL0 
Go to UniProtKB:  Q9QZL0
IMPC:  MGI:2154952
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ9QZL0
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLID-STATE NMR
  • Conformers Calculated: 200 
  • Conformers Submitted: 10 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
Other governmentChinaMinistry of Science and Technology (China) 2017YFA0504804

Revision History  (Full details and data files)

  • Version 1.0: 2020-10-28
    Type: Initial release
  • Version 1.1: 2021-03-24
    Changes: Database references
  • Version 1.2: 2023-06-14
    Changes: Database references, Other
  • Version 1.3: 2024-05-15
    Changes: Data collection, Database references