6Z3W

Human ER membrane protein complex


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 6.40 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation   3D Report Full Report


This is version 1.1 of the entry. See complete history

Re-refinement Note

This entry reflects an alternative modeling of the original data in: 6Y4L


Literature

The architecture of EMC reveals a path for membrane protein insertion.

O'Donnell, J.P.Phillips, B.P.Yagita, Y.Juszkiewicz, S.Wagner, A.Malinverni, D.Keenan, R.J.Miller, E.A.Hegde, R.S.

(2020) Elife 9

  • DOI: https://doi.org/10.7554/eLife.57887
  • Primary Citation of Related Structures:  
    6Y4L, 6Z3W

  • PubMed Abstract: 

    Approximately 25% of eukaryotic genes code for integral membrane proteins that are assembled at the endoplasmic reticulum. An abundant and widely conserved multi-protein complex termed EMC has been implicated in membrane protein biogenesis, but its mechanism of action is poorly understood. Here, we define the composition and architecture of human EMC using biochemical assays, crystallography of individual subunits, site-specific photocrosslinking, and cryo-EM reconstruction. Our results suggest that EMC's cytosolic domain contains a large, moderately hydrophobic vestibule that can bind a substrate's transmembrane domain (TMD). The cytosolic vestibule leads into a lumenally-sealed, lipid-exposed intramembrane groove large enough to accommodate a single substrate TMD. A gap between the cytosolic vestibule and intramembrane groove provides a potential path for substrate egress from EMC. These findings suggest how EMC facilitates energy-independent membrane insertion of TMDs, explain why only short lumenal domains are translocated by EMC, and constrain models of EMC's proposed chaperone function.


  • Organizational Affiliation

    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
ER membrane protein complex subunit 1,ER membrane protein complex subunit 1,ER membrane protein complex subunit 1,ER membrane protein complex subunit 1993Homo sapiensMutation(s): 0 
Gene Names: EMC1KIAA0090PSEC0263
Membrane Entity: Yes 
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Find proteins for Q8N766 (Homo sapiens)
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Go to UniProtKB:  Q8N766
PHAROS:  Q8N766
GTEx:  ENSG00000127463 
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UniProt GroupQ8N766
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
ER membrane protein complex subunit 2297Homo sapiensMutation(s): 0 
Gene Names: EMC2KIAA0103TTC35
Membrane Entity: Yes 
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Find proteins for Q15006 (Homo sapiens)
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PHAROS:  Q15006
GTEx:  ENSG00000104412 
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UniProt GroupQ15006
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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
ER membrane protein complex subunit 3261Homo sapiensMutation(s): 0 
Gene Names: EMC3TMEM111
Membrane Entity: Yes 
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PHAROS:  Q9P0I2
GTEx:  ENSG00000125037 
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UniProt GroupQ9P0I2
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Entity ID: 4
MoleculeChains Sequence LengthOrganismDetailsImage
ER membrane protein complex subunit 4183Homo sapiensMutation(s): 0 
Gene Names: EMC4TMEM85HSPC184PIG17
Membrane Entity: Yes 
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Find proteins for Q5J8M3 (Homo sapiens)
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PHAROS:  Q5J8M3
GTEx:  ENSG00000128463 
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UniProt GroupQ5J8M3
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Entity ID: 5
MoleculeChains Sequence LengthOrganismDetailsImage
Membrane magnesium transporter 1131Homo sapiensMutation(s): 0 
Gene Names: MMGT1EMC5TMEM32
Membrane Entity: Yes 
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GTEx:  ENSG00000169446 
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UniProt GroupQ8N4V1
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Entity ID: 6
MoleculeChains Sequence LengthOrganismDetailsImage
ER membrane protein complex subunit 6110Homo sapiensMutation(s): 0 
Gene Names: EMC6TMEM93
Membrane Entity: Yes 
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GTEx:  ENSG00000127774 
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UniProt GroupQ9BV81
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Entity ID: 7
MoleculeChains Sequence LengthOrganismDetailsImage
ER membrane protein complex subunit 7242Homo sapiensMutation(s): 0 
Gene Names: EMC7C11orf3C15orf24HT022UNQ905/PRO1926
Membrane Entity: Yes 
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GTEx:  ENSG00000134153 
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Entity ID: 8
MoleculeChains Sequence LengthOrganismDetailsImage
ER membrane protein complex subunit 9208Homo sapiensMutation(s): 0 
Gene Names: EMC9C14orf122FAM158ACGI-112
Membrane Entity: Yes 
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Find proteins for Q9Y3B6 (Homo sapiens)
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GTEx:  ENSG00000100908 
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UniProt GroupQ9Y3B6
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Entity ID: 9
MoleculeChains Sequence LengthOrganismDetailsImage
ER membrane protein complex subunit 10262Homo sapiensMutation(s): 0 
Gene Names: EMC10C19orf63HSM1INM02UNQ764/PRO1556
Membrane Entity: Yes 
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Find proteins for Q5UCC4 (Homo sapiens)
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PHAROS:  Q5UCC4
GTEx:  ENSG00000161671 
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UniProt GroupQ5UCC4
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Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 6.40 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

Structure Validation

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Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
Medical Research Council (MRC, United Kingdom)United KingdomMC_UP_A022_1007
European Molecular Biology Organization (EMBO)GermanyALTF-18-2018

Revision History  (Full details and data files)

  • Version 1.0: 2020-07-15
    Type: Initial release
  • Version 1.1: 2024-05-15
    Changes: Data collection, Database references