6K4W

smart chimeric peptide SCP-A6

  • Classification: ANTIBIOTIC
  • Organism(s): Arenicola marina
  • Mutation(s): No 

  • Deposited: 2019-05-27 Released: 2019-06-12 
  • Deposition Author(s): Wang, J.H., Liu, X.H.
  • Funding Organization(s): National Natural Science Foundation of China

Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Development of chimeric peptides to facilitate the neutralisation of lipopolysaccharides during bactericidal targeting of multidrug-resistant Escherichia coli.

Wang, Z.Liu, X.Mao, R.Hao, Y.Yang, N.Wang, X.Li, Z.Wang, X.Wang, J.

(2020) Commun Biol 3: 41-41

  • DOI: https://doi.org/10.1038/s42003-020-0761-3
  • Primary Citation of Related Structures:  
    6K4V, 6K4W

  • PubMed Abstract: 

    Pathogenic Escherichia coli can cause fatal diarrheal diseases in both animals and humans. However, no antibiotics or antimicrobial peptides (AMPs) can adequately kill resistant bacteria and clear bacterial endotoxin, lipopolysaccharide (LPS) which leads to inflammation and sepsis. Here, the LPS-targeted smart chimeric peptides (SCPs)-A6 and G6 are generated by connecting LPS-targeting peptide-LBP14 and killing domain-N6 via different linkers. Rigid and flexible linkers retain the independent biological activities from each component. SCPs-A6 and G6 exert low toxicity and no bacterial resistance, and they more rapidly kill multiple-drug-resistant E. coli and more effectively neutralize LPS toxicity than N6 alone. The SCPs can enhance mouse survival more effectively than N6 or polymyxin B and alleviate lung injuries by blocking mitogen-activated protein kinase and nuclear factor kappa-B p65 activation. These findings uniquely show that SCPs-A6 and G6 may be promising dual-function candidates as improved antibacterial and anti-endotoxin agents to treat bacterial infection and sepsis.


  • Organizational Affiliation

    Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
SCP-A645Arenicola marinaMutation(s): 0 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Natural Science Foundation of ChinaChina31570061
National Natural Science Foundation of ChinaChina31772640
National Natural Science Foundation of ChinaChina31572444
National Natural Science Foundation of ChinaChina31372346
National Natural Science Foundation of ChinaChina31672467
National Natural Science Foundation of ChinaChina31572445

Revision History  (Full details and data files)

  • Version 1.0: 2019-06-12
    Type: Initial release
  • Version 1.1: 2020-12-23
    Changes: Database references
  • Version 1.2: 2023-06-14
    Changes: Database references, Other
  • Version 1.3: 2024-05-15
    Changes: Data collection, Database references