6ELQ

Experimental Data Snapshot

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

(2017) Angew Chem Int Ed Engl 56: 15466-15469

PubMed Abstract:
CO dehydrogenases (CODHs) catalyse the reversible conversion between CO and CO ₂ . Genomic analysis indicated that the metabolic functions of CODHs vary. The genome of Carboxydothermus hydrogenoformans encodes five CODHs (CODH-I-V), of which CODH-IV is found in a gene cluster near a peroxide-reducing enzyme. Our kinetic and crystallographic experiments reveal that CODH-IV differs from other CODHs in several characteristic properties: it has a very high affinity for CO, oxidizes CO at diffusion-limited rate over a wide range of temperatures, and is more tolerant to oxygen than CODH-II. Thus, our observations support the idea that CODH-IV is a CO scavenger in defence against oxidative stress and highlight that CODHs are more diverse in terms of reactivity than expected.

Organizational Affiliation

Institut für Biologie, Strukturbiologie/Biochemie, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099, Berlin, Germany.

Macromolecule Content

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Carbon Monoxide Dehydrogenase	A [auth X], B [auth A], C [auth B]	633	Carboxydothermus hydrogenoformans	Mutation(s): 0 Gene Names: cooSIV EC: 1.2.7.4
UniProt
Find proteins for Q3AE44 (Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)) Explore Q3AE44 Go to UniProtKB: Q3AE44
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q3AE44
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 2 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
BF8 Query on BF8 Download Ideal Coordinates CCD File SDF format, chain E [auth X] SDF format, chain H [auth A] SDF format, chain K [auth B] MOL2 format, chain E [auth X] MOL2 format, chain H [auth A] MOL2 format, chain K [auth B]	E [auth X], H [auth A], K [auth B]	FE(4)-NI(1)-S(5) CLUSTER with Oxygen Fe₄ Ni O S₄ DGNZLMZPCMYVGP-UHFFFAOYSA-N		Interactions Focus chain E [auth X] Focus chain H [auth A] Focus chain K [auth B] Interactions & Density Focus chain E [auth X] Focus chain H [auth A] Focus chain K [auth B]
SF4 Query on SF4 Download Ideal Coordinates CCD File SDF format, chain D [auth X] SDF format, chain F [auth A] SDF format, chain G [auth A] SDF format, chain I [auth B] SDF format, chain J [auth B] MOL2 format, chain D [auth X] MOL2 format, chain F [auth A] MOL2 format, chain G [auth A] MOL2 format, chain I [auth B] MOL2 format, chain J [auth B]	D [auth X], F [auth A], G [auth A], I [auth B], J [auth B]	IRON/SULFUR CLUSTER Fe₄ S₄ LJBDFODJNLIPKO-UHFFFAOYSA-N		Interactions Focus chain D [auth X] Focus chain F [auth A] Focus chain G [auth A] Focus chain I [auth B] Focus chain J [auth B] Interactions & Density Focus chain D [auth X] Focus chain F [auth A] Focus chain G [auth A] Focus chain I [auth B] Focus chain J [auth B]