5Z1G

Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate

(2019) Protein Cell 10: 120-130

• PubMed: 29557065 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1007/s13238-018-0526-7
• Primary Citation of Related Structures:  
  5Z1G, 5Z3G


• PubMed Abstract: 
  

  Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits.

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Organizational Affiliation: 

Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, China.