4IH1

Crystal structure of Karrikin Insensitive 2 (KAI2) from Arabidopsis thaliana

PDB DOI: https://doi.org/10.2210/pdb4IH1/pdb

Classification: HYDROLASE
Organism(s): Arabidopsis thaliana
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2012-12-18 Released: 2013-01-30
Deposition Author(s): Zhou, X.E., Zhao, L.-H., Wu, Z.-S., Yi, W., Li, S., Li, Y., Xu, Y., Xu, T.-H., Liu, Y., Chen, R.-Z., Kovach, A., Kang, Y., Hou, L., He, Y., Zhang, C., Melcher, K., Xu, H.E.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.55 Å
R-Value Free: 0.193
R-Value Work: 0.179
R-Value Observed: 0.180

wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

Crystal structures of two phytohormone signal-transducing alpha / beta hydrolases: karrikin-signaling KAI2 and strigolactone-signaling DWARF14.

Zhao, L.H., Zhou, X.E., Wu, Z.S., Yi, W., Xu, Y., Li, S., Xu, T.H., Liu, Y., Chen, R.Z., Kovach, A., Kang, Y., Hou, L., He, Y., Xie, C., Song, W., Zhong, D., Xu, Y., Wang, Y., Li, J., Zhang, C., Melcher, K., Xu, H.E.

(2013) Cell Res 23: 436-439

PubMed: 23381136 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1038/cr.2013.19
Primary Citation of Related Structures:
4IH1, 4IH4, 4IH9, 4IHA

Macromolecule Content

Total Structure Weight: 29.82 kDa
Atom Count: 2,196
Modelled Residue Count: 268
Deposited Residue Count: 270
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Hydrolase, alpha/beta fold family protein	A	270	Arabidopsis thaliana	Mutation(s): 0 Gene Names: AT4g37470, F6G17.120, kai2
UniProt
Find proteins for Q9SZU7 (Arabidopsis thaliana) Explore Q9SZU7 Go to UniProtKB: Q9SZU7
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9SZU7
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.55 Å
R-Value Free: 0.193
R-Value Work: 0.179
R-Value Observed: 0.180
Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 51.335	α = 90
b = 56.013	β = 116.15
c = 53.525	γ = 90

Software Package:

Software Name	Purpose
SCALEPACK	data scaling
REFMAC	refinement
PDB_EXTRACT	data extraction
HKL-2000	data reduction

Structure Validation

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Entry History

Deposition Data

Released Date: 2013-01-30

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2013-01-30
Type: Initial release
Version 1.1: 2013-10-23
Changes: Database references
Version 1.2: 2023-09-20
Changes: Data collection, Database references, Refinement description

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Help and Resources

4IH1

Crystal structure of Karrikin Insensitive 2 (KAI2) from Arabidopsis thaliana

Crystal structures of two phytohormone signal-transducing alpha / beta hydrolases: karrikin-signaling KAI2 and strigolactone-signaling DWARF14.

Entity ID: 1

UniProt

Entity Groups

Sequence Annotations

Expand

Experimental Data

Structure Validation

Deposition Data

Revision History (Full details and data files)