2N2Y

Solution structure of the meiosis-expressed gene 1 (Meig1)


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 50 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Dissecting the structural basis of MEIG1 interaction with PACRG.

Li, W.Walavalkar, N.M.Buchwald, W.A.Teves, M.E.Zhang, L.Liu, H.Bilinovich, S.Peterson, D.L.Strauss Iii, J.F.Williams, D.C.Zhang, Z.

(2016) Sci Rep 6: 18278-18278

  • DOI: https://doi.org/10.1038/srep18278
  • Primary Citation of Related Structures:  
    2N2Y

  • PubMed Abstract: 

    The product of the meiosis-expressed gene 1 (MEIG1) is found in the cell bodies of spermatocytes and recruited to the manchette, a structure unique to elongating spermatids, by Parkin co-regulated gene (PACRG). This complex is essential for targeting cargo to the manchette during sperm flagellum assembly. Here we show that MEIG1 adopts a unique fold that provides a large surface for interacting with other proteins. We mutated 12 exposed and conserved amino acids and show that four of these mutations (W50A, K57E, F66A, Y68A) dramatically reduce binding to PACRG. These four amino acids form a contiguous hydrophobic patch on one end of the protein. Furthermore, each of these four mutations diminishes the ability of MEIG1 to stabilize PACRG when expressed in bacteria. Together these studies establish the unique structure and key interaction surface of MEIG1 and provide a framework to explore how MEIG1 recruits proteins to build the sperm tail.


  • Organizational Affiliation

    Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, 23298.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Meiosis-expressed gene 1 protein92Mus musculusMutation(s): 0 
Gene Names: Meig1Meg1
UniProt
Find proteins for Q61845 (Mus musculus)
Explore Q61845 
Go to UniProtKB:  Q61845
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ61845
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 50 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2016-01-20
    Type: Initial release
  • Version 1.1: 2023-06-14
    Changes: Data collection, Database references, Other
  • Version 1.2: 2024-05-15
    Changes: Data collection, Database references