2MMV

ZapA mutant dimer from Geobacillus stearothermophilus


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the least restraint violations 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Backbone and side chain NMR assignments of Geobacillus stearothermophilus ZapA allow identification of residues that mediate the interaction of ZapA with FtsZ.

Nogueira, M.L.Sforca, M.L.Chin, Y.K.Mobli, M.Handler, A.Gorbatyuk, V.Y.Robson, S.A.King, G.F.Gueiros-Filho, F.J.Zeri, A.C.

(2015) Biomol NMR Assign 9: 387-391

  • DOI: https://doi.org/10.1007/s12104-015-9615-1
  • Primary Citation of Related Structures:  
    2MMV

  • PubMed Abstract: 

    Bacterial division begins with the formation of a contractile protein ring at midcell, which constricts the bacterial envelope to generate two daughter cells. The central component of the division ring is FtsZ, a tubulin-like protein capable of self-assembling into filaments which further associate into a higher order structure known as the Z ring. Proteins that bind to FtsZ play a crucial role in the formation and regulation of the Z ring. One such protein is ZapA, a widely conserved 21 kDa homodimeric protein that associates with FtsZ filaments and promotes their bundling. Although ZapA was discovered more than a decade ago, the structural details of its interaction with FtsZ remain unknown. In this work, backbone and side chain NMR assignments for the Geobacillus stearothermophilus ZapA homodimer are described. We titrated FtsZ into (15)N(2)H-ZapA and mapped ZapA residues whose resonances are perturbed upon FtsZ binding. This information provides a structural understanding of the interaction between FtsZ and ZapA.


  • Organizational Affiliation

    Brazilian Biosciences National Laboratory (LNBio-CNPEM), Campinas, Brazil. marialuiza.nogueira@lnbio.cnpem.br.


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Cell division protein ZapA
A, B
86Geobacillus stearothermophilusMutation(s): 3 
Gene Names: zapa
UniProt
Find proteins for A0A078N0N2 (Geobacillus stearothermophilus)
Explore A0A078N0N2 
Go to UniProtKB:  A0A078N0N2
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupA0A078N0N2
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the least restraint violations 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2015-06-17
    Type: Initial release
  • Version 1.1: 2022-12-14
    Changes: Data collection, Database references
  • Version 1.2: 2023-06-14
    Changes: Other
  • Version 1.3: 2024-05-15
    Changes: Data collection, Database references