2LOR

Backbone structure of human membrane protein TMEM141


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 200 
  • Conformers Submitted: 20 
  • Selection Criteria: target function 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Facile backbone structure determination of human membrane proteins by NMR spectroscopy.

Klammt, C.Maslennikov, I.Bayrhuber, M.Eichmann, C.Vajpai, N.Chiu, E.J.Blain, K.Y.Esquivies, L.Kwon, J.H.Balana, B.Pieper, U.Sali, A.Slesinger, P.A.Kwiatkowski, W.Riek, R.Choe, S.

(2012) Nat Methods 9: 834-839

  • DOI: https://doi.org/10.1038/nmeth.2033
  • Primary Citation of Related Structures:  
    2LOM, 2LON, 2LOO, 2LOP, 2LOQ, 2LOR, 2LOS

  • PubMed Abstract: 

    Although nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight <30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization.


  • Organizational Affiliation

    Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Transmembrane protein 141108Homo sapiensMutation(s): 0 
Gene Names: TMEM141
Membrane Entity: Yes 
UniProt & NIH Common Fund Data Resources
Find proteins for Q96I45 (Homo sapiens)
Explore Q96I45 
Go to UniProtKB:  Q96I45
PHAROS:  Q96I45
GTEx:  ENSG00000244187 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ96I45
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 200 
  • Conformers Submitted: 20 
  • Selection Criteria: target function 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2012-05-23
    Type: Initial release
  • Version 1.1: 2012-08-15
    Changes: Database references
  • Version 1.2: 2023-06-14
    Changes: Data collection, Database references, Other
  • Version 1.3: 2024-05-15
    Changes: Data collection, Database references