1NZ8

Solution Structure of the N-utilization substance G (NusG) N-terminal (NGN) domain from Thermus thermophilus


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 90 
  • Conformers Submitted: 31 
  • Selection Criteria: structures with the lowest energy, NONE WITH DISTANCE VIOLATIONS > 0.2 ANGSTROMS, NONE WITH DIHEDRAL ANGLE RESTRAINT VIOLATIONS > 2 DEGREES 

wwPDB Validation   3D Report Full Report


This is version 1.4 of the entry. See complete history


Literature

Structural and sequence comparisons arising from the solution structure of the transcription elongation factor NusG from Thermus thermophilus

Reay, P.Yamasaki, K.Terada, T.Kuramitsu, S.Shirouzu, M.Yokoyama, S.

(2004) Proteins 56: 40-51

  • DOI: https://doi.org/10.1002/prot.20054
  • Primary Citation of Related Structures:  
    1NZ8, 1NZ9

  • PubMed Abstract: 

    NusG is an essential bacterial protein modulator of transcriptional elongation and termination events, and interacts directly with RNA polymerase and Rho protein. Found also in Archaea, NusG shows stretches of sequence similarity to the eukaryotic transcription elongation factor Spt5. Herein, the three-dimensional solution structure of the bacterial NusG from Thermus thermophilus, which shows 43% amino acid sequence similarity to the Escherichia coli NusG, is described, and a survey of NusG and Spt5 amino acid sequences is presented. Although there is a clear evolutionary and functional relationship between these proteins, it is evident from the structural, sequence, and biochemical data that their binding specificities to both nucleic acids and other proteins differ.


  • Organizational Affiliation

    Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
TRANSCRIPTION ANTITERMINATION PROTEIN NUSG119Thermus thermophilusMutation(s): 0 
Gene Names: NusG
UniProt
Find proteins for P35872 (Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8))
Explore P35872 
Go to UniProtKB:  P35872
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP35872
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 90 
  • Conformers Submitted: 31 
  • Selection Criteria: structures with the lowest energy, NONE WITH DISTANCE VIOLATIONS > 0.2 ANGSTROMS, NONE WITH DIHEDRAL ANGLE RESTRAINT VIOLATIONS > 2 DEGREES 

Structure Validation

View Full Validation Report



Entry History 

Revision History  (Full details and data files)

  • Version 1.0: 2004-04-06
    Type: Initial release
  • Version 1.1: 2008-04-29
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2013-10-30
    Changes: Derived calculations
  • Version 1.4: 2024-05-01
    Changes: Data collection, Database references