1D8K

SOLUTION STRUCTURE OF THE CENTRAL CORE DOMAIN OF TFIIE BETA


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 
  • Selection Criteria: LEAST RESTRAINT VIOLATIONS 

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This is version 1.4 of the entry. See complete history


Literature

Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface.

Okuda, M.Watanabe, Y.Okamura, H.Hanaoka, F.Ohkuma, Y.Nishimura, Y.

(2000) EMBO J 19: 1346-1356

  • DOI: https://doi.org/10.1093/emboj/19.6.1346
  • Primary Citation of Related Structures:  
    1D8J, 1D8K

  • PubMed Abstract: 

    Human general transcription factor TFIIE consists of two subunits, TFIIEalpha and TFIIEbeta. Recently, TFIIEbeta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. Here, the central core domain of human TFIIEbeta (TFIIEbetac) has been identified by a limited proteolysis. This solution structure has been determined by NMR. It consists of three helices with a beta hairpin at the C-terminus, resembling the winged helix proteins. However, TFIIEbetac shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow. A model will be proposed that TFIIE stabilizes the preinitiation complex by binding not only to the general transcription factors together with RNA polymerase II but also to the promoter DNA, where double-stranded DNA starts to open to be single-stranded upon activation of the preinitiation complex.


  • Organizational Affiliation

    Graduate School of Integrated Science, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, USA.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
GENERAL TRANSCRIPTION FACTOR TFIIE-BETA81Homo sapiensMutation(s): 0 
UniProt & NIH Common Fund Data Resources
Find proteins for P29084 (Homo sapiens)
Explore P29084 
Go to UniProtKB:  P29084
PHAROS:  P29084
GTEx:  ENSG00000197265 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP29084
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 
  • Selection Criteria: LEAST RESTRAINT VIOLATIONS 

Structure Validation

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Entry History 

Revision History  (Full details and data files)

  • Version 1.0: 2000-04-26
    Type: Initial release
  • Version 1.1: 2008-04-27
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2022-02-16
    Changes: Data collection, Database references, Derived calculations
  • Version 1.4: 2024-05-22
    Changes: Data collection