Auxilin

UniProtKB accession:  Q27974

Grouped By:  Matching UniProtKB accession

UniProtKB description:  May act as a protein phosphatase and/or a lipid phosphatase (Probable). Co-chaperone that recruits HSPA8/HSC70 to clathrin-coated vesicles (CCVs) and promotes the ATP-dependent dissociation of clathrin from CCVs and participates in clathrin-mediated endocytosis of synaptic vesicles and their recycling and also in intracellular trafficking (PubMed:8524399, PubMed:11470803). Firstly, binds tightly to the clathrin cages, at a ratio of one DNAJC6 per clathrin triskelion (PubMed:21482805, PubMed:7705342, PubMed:15023062, PubMed:8524399, PubMed:15502813). The HSPA8:ATP complex then binds to the clathrin-auxilin cage, initially at a ratio of one HSPA8 per triskelion leading to ATP hydrolysis stimulation and causing a conformational change in the HSPA8 (PubMed:21482805, PubMed:15023062, PubMed:8524399, PubMed:17996706). This cycle is repeated three times to drive to a complex containing the clathrin-auxilin cage associated to three HSPA8:ADP complex (PubMed:21482805). The ATP hydrolysis of the third HSPA8:ATP complex leads to a concerted dismantling of the cage into component triskelia (PubMed:21482805). Then, dissociates from the released triskelia and be recycled to initiate another cycle of HSPA8's recruitment (PubMed:21482805, PubMed:8524399). Also acts during the early steps of clathrin-coated vesicle (CCV) formation through its interaction with the GTP bound form of DNM1 (PubMed:12791276).