Crossover junction endodeoxyribonuclease RuvC

UniProtKB accession:  P0A814

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UniProtKB description:  The RuvA-RuvB-RuvC complex processes Holliday junctions during genetic recombination and DNA repair (PubMed:6374379). Endonuclease that resolves Holliday junction (HJ) intermediates. Cleaves cruciform DNA by making single-stranded nicks across the junction at symmetrical positions within the homologous arms, leaving a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction (PubMed:1661673, PubMed:1758493, PubMed:1829835, PubMed:8001122, PubMed:8106500, PubMed:8195150, PubMed:9000618, PubMed:9160752, PubMed:9135161, PubMed:10471285, PubMed:36000732). The consensus cleavage sequence is 5'-(A/T)TT(C>G/A)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange, although there is some flexibility in the position cleaved (PubMed:8001122, PubMed:8195150, PubMed:9135161, PubMed:10471285). The cleavage reactions can be uncoupled; incision requires the presence of two consensus cleavage sequences, although they do not have to be identical (PubMed:9135161). The presence of a 5'-phosphate in a half-cut site accelerates cleavage of the second site, ensuring the second cleavage occurs within the lifetime of a single RuvC-HJ complex (PubMed:19399178). Binds to cruciform DNA in a sequence non-specific manner (PubMed:8195150, PubMed:8106500, PubMed:10471285).