LINE-1 retrotransposable element ORF2 protein

UniProtKB accession:  O00370

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UniProtKB description:  Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition (PubMed:9140393, PubMed:7516468). Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA (PubMed:8945517, PubMed:17626046, PubMed:34554261). Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome (PubMed:8945517). Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition (PubMed:17626046, PubMed:34554261). Unlike related endonucleases, does not bend the DNA helix but causes compression near the cleavage site (PubMed:34554261).