L-evernosamine nitrososynthase

UniProtKB accession:  C4RPA1

Grouped By:  Matching UniProtKB accession

UniProtKB description:  Nitrososynthase involved in the biosynthesis of everninomicin, a broad spectrum orthosomycin antibiotic (PubMed:18983146). Catalyzes the double-oxidation of TDP-L-evernosamine to TDP-L-evernitrosose (PubMed:18983146, PubMed:20866105, PubMed:23885759). The enzyme first oxidizes the substrate to a transient hydroxylamino intermediate, which is then further oxidized to nitroso sugar (PubMed:18983146, PubMed:20866105, PubMed:23885759). The nitroso group is probably spontaneously oxidized giving TDP-L-evernitrose (PubMed:18983146). In vitro, catalyzes the double-oxidation of TDP-L-epi-vancosamine to TDP-L-epi-vancosonitrose (PubMed:18983146, PubMed:20866105, PubMed:23885759). Can also use biosynthetic progenitors of TDP-L-epi-vancosamine, but progenitors solely undergo single-oxidation reactions and terminate in the hydroxylamine oxidation state (PubMed:20866105).