8I48

Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in closed state


ELECTRON MICROSCOPY

Refinement

RMS Deviations
KeyRefinement Restraint Deviation
f_dihedral_angle_d16.18
f_angle_d0.622
f_chiral_restr0.044
f_plane_restr0.005
f_bond_d0.004
Sample
pentameric ligand-gated ion channel
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles254591
Reported Resolution (Å)2.74
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryC5
Map-Model Fitting and Refinement
Id1
Refinement Space
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeFEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å**2)71
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)600
Maximum Defocus (nm)1400
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
PARTICLE SELECTIONcryoSPARC4.2.1
IMAGE ACQUISITIONEPU
CTF CORRECTIONCTFFIND4.1
MODEL FITTINGCoot0.9.8.7
MODEL REFINEMENTPHENIX1.21
INITIAL EULER ASSIGNMENTRELION4.0.1
FINAL EULER ASSIGNMENTRELION4.0.1
CLASSIFICATIONRELION4.0.1
RECONSTRUCTIONRELION4.0.1
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION641067