7JIY
Structure of truncated zebrafish paragranulin
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-1H TOCSY | 0.2 mM ZF-para_3s | 90% H2O/10% D2O | 0 M | 3 | ambient Pa | 290 | Bruker AVANCE 600 |
| 2 | 2D 1H-1H NOESY | 0.2 mM ZF-para_3s | 90% H2O/10% D2O | 0 M | 3 | ambient Pa | 290 | Bruker AVANCE 600 |
| 3 | 2D 1H-1H COSY | 0.2 mM ZF-para_3s | 90% H2O/10% D2O | 0 M | 3 | ambient Pa | 290 | Bruker AVANCE 600 |
| 4 | 2D 1H-13C HSQC | 0.2 mM ZF-para_3s | 90% H2O/10% D2O | 0 M | 3 | ambient Pa | 290 | Bruker AVANCE 600 |
| 5 | 2D 1H-15N HSQC | 0.2 mM ZF-para_3s | 90% H2O/10% D2O | 0 M | 3 | ambient Pa | 290 | Bruker AVANCE 600 |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | CYANA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | target function |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | TopSpin | Bruker Biospin | |
| 5 | structure calculation | CYANA | Guntert, Mumenthaler and Wuthrich | |
| 3 | chemical shift assignment | CcpNmr Analysis | CCPN | |
