7F2O
Cryo-EM structure of the type 2 bradykinin receptor in complex with the bradykinin and an Gq protein
ELECTRON MICROSCOPY
Refinement
| RMS Deviations | |
|---|---|
| Key | Refinement Restraint Deviation |
| f_dihedral_angle_d | 21.802 |
| f_angle_d | 0.55 |
| f_chiral_restr | 0.042 |
| f_plane_restr | 0.005 |
| f_bond_d | 0.002 |
| Sample |
|---|
| Cryo-EM structure of the type 2 bradykinin receptor in complex with the bradykinin and an Gq protein |
| Sample Components |
| G subunit q (Gi2-mini-Gq chimeric) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
| ARG-PRO-PRO-GLY-PHE-SER-PRO-PHE-ARG |
| Type 2 bradykinin receptor |
| single Fab chain (svFv16) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 664416 |
| Reported Resolution (Å) | 2.9 |
| Resolution Method | FSC 0.143 CUT-OFF |
| Other Details | |
| Refinement Type | |
| Symmetry Type | POINT |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 | ||||
| Refinement Space | |||||
| Refinement Protocol | |||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | |||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | OTHER | ||||||||
| Electron Dose (electrons/Å**2) | 80 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | |
| Temperature (Kelvin) | |
| Microscope Model | FEI TITAN KRIOS |
| Minimum Defocus (nm) | |
| Maximum Defocus (nm) | |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | |
| Calibrated Magnification | |
| Source | OTHER |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| NONE | ||||
