NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 3D 1H-15N TOCSY | 1 mM [U-99% 15N] LicT CAT-PRD1 mutant, 200 mM sodium chloride, 0.5 mM EDTA, 0.2 mM Benzamidine, 10 mM TRIS, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 8 | 1 atm | 308 | Bruker AVANCE III 800 |
2 | 3D 1H-15N NOESY | 1 mM [U-99% 15N] LicT CAT-PRD1 mutant, 200 mM sodium chloride, 0.5 mM EDTA, 0.2 mM Benzamidine, 10 mM TRIS, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 8 | 1 atm | 308 | Bruker AVANCE III 800 |
3 | 3D CBCA(CO)NH | 0.8 mM [U-99% 13C; U-99% 15N] LicT CAT-PRD1 mutant, 10 mM sodium phosphate, 200 mM NaCl, 0.2 mM EDTA, 0.2 mM DTT, 0.2 mM benzamidine | 90% H2O/10% D2O | 200 mM | 6.4 | 1 atm | 303 | Bruker AVANCE III 800 |
4 | 3D HN(CO)CA | 0.8 mM [U-99% 13C; U-99% 15N] LicT CAT-PRD1 mutant, 200 mM sodium chloride, 10 mM TRIS, 0.5 mM EDTA, 0.2 mM benzamidine, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 6.4 | 1 atm | 303 | Bruker AVANCE III 800 |
5 | 3D HNCA | 0.8 mM [U-99% 13C; U-99% 15N] LicT CAT-PRD1 mutant, 10 mM sodium phosphate, 200 mM NaCl, 0.2 mM EDTA, 0.2 mM DTT, 0.2 mM benzamidine | 90% H2O/10% D2O | 200 mM | 6.4 | 1 atm | 303 | Bruker AVANCE III 800 |
6 | 2D 1H-13C Filtered NOESY | 0.380 mM [U-99% 13C; U-99% 15N] CAT-PRD1 mutant, 200 mM sodium chloride, 10 mM NA TRIS, 0.2 mM EDTA, 0.2 mM Benzamidine, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 6.4 | 1 atm | 303 | Bruker AVANCE III 800 |
7 | 3D HNCO | 0.8 mM [U-99% 13C; U-99% 15N] LicT CAT-PRD1 mutant, 200 mM sodium chloride, 10 mM TRIS, 0.5 mM EDTA, 0.2 mM benzamidine, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 6.4 | 1 atm | 303 | Bruker AVANCE III 800 |
8 | 3D HNCA | 0.8 mM [U-99% 13C; U-99% 15N] LicT CAT-PRD1 mutant, 200 mM sodium chloride, 10 mM TRIS, 0.5 mM EDTA, 0.2 mM benzamidine, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 8 | 1 atm | 308 | Bruker AVANCE III 800 |
9 | 3D CBCA(CO)NH | 0.8 mM [U-99% 13C; U-99% 15N] LicT CAT-PRD1 mutant, 200 mM sodium chloride, 10 mM TRIS, 0.5 mM EDTA, 0.2 mM benzamidine, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 8 | 1 atm | 308 | Bruker AVANCE III 800 |
10 | 3D HNCACB | 0.8 mM [U-99% 13C; U-99% 15N] LicT CAT-PRD1 mutant, 200 mM sodium chloride, 10 mM TRIS, 0.5 mM EDTA, 0.2 mM benzamidine, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 8 | 1 atm | 308 | Bruker AVANCE III 800 |
11 | 3D HCCH-TOCSY | 0.8 mM [U-99% 13C; U-99% 15N] LicT CAT-PRD1 mutant, 200 mM sodium chloride, 10 mM TRIS, 0.5 mM EDTA, 0.2 mM benzamidine, 1 mM DTT | 90% H2O/10% D2O | 200 mM | 8 | 1 atm | 308 | Bruker AVANCE III 800 |