NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
2 | 2D 1H-13C HSQC aliphatic | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
3 | 2D 1H-13C HSQC aromatic | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
4 | 2D 1H-13C HSQC aliphatic constant-time | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
5 | 2D 1H-1H NOESY (150ms mixing time) filtered to reject 13C-1 and 15N-coupled 1H in F1 and to accept 13C- and 15N-coupled 1H in F2 | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
15 | 3D HNCA | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
14 | 3D HN(CO)CA | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
13 | 3D CBCANH | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
12 | 3D CBCA(CO)NH | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
11 | 3D HBHA(CO)NH | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
10 | 3D HCCH-TOCSY [1H-13C-1H] | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
9 | 3D HCCH-TOCSY [13C-13C-1H] | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
8 | 3D 15N NOESY-HSQC (150ms mixing time) | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
7 | 3D 13C NOESY-HSQC aliphatic (150ms mixing time) | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
6 | 3D 13C NOESY-HSQC aromatic (150ms mixing time) | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
19 | 2D 1H-13C HSQC aliphatic | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
18 | 2D 1H-13C HSQC aromatic | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
17 | 2D 1H-13C HSQC aliphatic constant-time | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
16 | 2D 1H-1H NOESY (150ms mixing time) filtered to reject 13C-1 and 15N-coupled 1H in F1 and to accept 13C- and 15N-coupled 1H in F2 | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
20 | 2D 1H-1H NOESY (70ms mixing time) filtered to reject 13C-1 and 15N-coupled 1H in F1 and to accept 13C- and 15N-coupled 1H in F2 | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
29 | 3D HCCH-TOCSY [13C-13C-1H] | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
28 | 3D 13C NOESY-HSQC (50ms mixing time) aliphatic | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
27 | 3D 13C NOESY-HSQC (120ms mixing time) aliphatic | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
26 | 3D 13C NOESY-HSQC (50ms mixing time) aromatic | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
25 | 3D 13C NOESY-HSQC (120ms mixing time) aromatic | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
24 | 3D 13C NOESY-HSQC (50ms mixing time) aliphatic, filtered to reject 13C-1 and 15N-coupled 1H in F1 | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
23 | 3D 13C NOESY-HSQC (120ms mixing time) aliphatic, filtered to reject 13C-1 and 15N-coupled 1H in F1 | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
22 | 3D 13C NOESY-HSQC (50ms mixing time) aromatic, filtered to reject 13C-1 and 15N-coupled 1H in F1 | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
21 | 3D 13C NOESY-HSQC (120ms mixing time) aromatic, filtered to reject 13C-1 and 15N-coupled 1H in F1 | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
30 | 2D 1H-15N HSQC | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
31 | 2D 1H-15N HSQC | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 283 | Bruker AVANCE 600 |
32 | 2D 1H-15N HSQC | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 275 | Bruker AVANCE 600 |
33 | 2D 1H-13C HSQC | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
34 | 3D CBCA(CO)NH | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
35 | 3D HNCA | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
36 | 3D HN(CO)CA | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
37 | 3D HBHA(CO)NH | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
38 | 3D HNHA | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
39 | 3D HCCH-TOCSY [1H-13C-1H] | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
40 | 3D HCCH-TOCSY [13C-13C-1H] | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
41 | 3D 1H-15N NOESY-HSQC (70ms mixing time) | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
42 | 3D 1H-15N NOESY-HSQC (150ms mixing time) | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
43 | 3D 1H-15N NOESY-HSQC (50ms mixing time) | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 283 | Bruker AVANCE 600 |
44 | 3D 1H-15N NOESY-HSQC (50ms mixing time) | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 275 | Bruker AVANCE III 800 |
45 | 3D 1H-13C NOESY (70ms mixing time) aliphatic | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 95% H2O/5% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
46 | 2D 1H-13C HSQC aliphatic | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
47 | 2D 1H-13C HSQC aromatic | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
48 | 3D HCCH-TOCSY [1H-13C-1H] | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
49 | 3D HCCH-TOCSY [13C-13C-1H] | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
50 | 3D 13C NOESY-HSQC aliphatic (50ms mixing time) | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
51 | 3D 13C NOESY-HSQC aliphatic (70ms mixing time) | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
52 | 3D 13C NOESY-HSQC aliphatic (120ms mixing time) | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |
53 | 3D 13C NOESY-HSQC aromatic (70ms mixing time) | 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE 600 |
54 | 2D 1H-13C HSQC aromatic constant-time | 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT | 100% D2O | 200 mM | 7.0 | 1 atm | 298 | Bruker AVANCE III 800 |