6PV2

Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: Orn in the metal-binding turn


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D 1H-1H TOCSY1.2 mM zinc finger 2 from transcription factor Sp1 DNA-binding domain, Orn turn variant, 10 mM [U-2H] TRIS, 0.05 mM DSS, 1.8 mM zinc chloride90% H2O/10% D2O10 mM61 atm298Bruker AVANCE 700
22D 1H-1H COSY1.2 mM zinc finger 2 from transcription factor Sp1 DNA-binding domain, Orn turn variant, 10 mM [U-2H] TRIS, 0.05 mM DSS, 1.8 mM zinc chloride90% H2O/10% D2O10 mM61 atm298Bruker AVANCE 700
32D 1H-1H NOESY1.2 mM zinc finger 2 from transcription factor Sp1 DNA-binding domain, Orn turn variant, 10 mM [U-2H] TRIS, 0.05 mM DSS, 1.8 mM zinc chloride90% H2O/10% D2O10 mM61 atm298Bruker AVANCE 700
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE700
NMR Refinement
MethodDetailsSoftware
simulated annealingARIA
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number100
Conformers Submitted Total Number10
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1processingTopSpinBruker Biospin
2data analysisSparkyGoddard
3refinementARIALinge, O'Donoghue and Nilges
4structure calculationARIALinge, O'Donoghue and Nilges