Experiment: 6IZG

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-15N HSQC	1.5 mM [U-13C; U-15N] Ufm1, 150 mM sodium chloride, 20 mM sodium phosphate, 2 mM EDTA, 2 mM DTT	90% H2O/10% D2O	150 mM	6.8	1 atm	298	Bruker DMX 600
2	3D 1H-15N NOESY	1.5 mM [U-13C; U-15N] Ufm1, 150 mM sodium chloride, 20 mM sodium phosphate, 2 mM EDTA, 2 mM DTT	90% H2O/10% D2O	150 mM	6.8	1 atm	298	Bruker DMX 600

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX	600

NMR Refinement
Method	Details	Software
simulated annealing		X-PLOR NIH

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	500
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	X-PLOR NIH		Schwieters, Kuszewski, Tjandra and Clore
2	chemical shift assignment	Sparky		Goddard
3	collection	NMRPipe		Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
4	structure calculation	CYANA		Guntert, Mumenthaler and Wuthrich

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.