6IFU

Cryo-EM structure of type III-A Csm-CTR2-dsDNA complex


ELECTRON MICROSCOPY

Refinement

RMS Deviations
KeyRefinement Restraint Deviation
r_dihedral_angle_2_deg40.827
r_long_range_B_refined30.272
r_long_range_B_other30.272
r_dihedral_angle_3_deg26.527
r_scangle_other25.511
r_dihedral_angle_4_deg23.318
r_mcangle_it20.665
r_mcangle_other20.665
r_dihedral_angle_1_deg17.635
r_scbond_it17.515
RMS Deviations
KeyRefinement Restraint Deviation
r_dihedral_angle_2_deg40.827
r_long_range_B_refined30.272
r_long_range_B_other30.272
r_dihedral_angle_3_deg26.527
r_scangle_other25.511
r_dihedral_angle_4_deg23.318
r_mcangle_it20.665
r_mcangle_other20.665
r_dihedral_angle_1_deg17.635
r_scbond_it17.515
r_scbond_other17.514
r_mcbond_it13.775
r_mcbond_other13.764
r_angle_refined_deg4.454
r_angle_other_deg2.143
r_chiral_restr0.257
r_bond_refined_d0.043
r_gen_planes_refined0.019
r_bond_other_d0.004
r_gen_planes_other0.004
r_nbd_refined
r_nbd_other
r_nbtor_refined
r_nbtor_other
r_xyhbond_nbd_refined
r_xyhbond_nbd_other
r_metal_ion_refined
r_metal_ion_other
r_symmetry_vdw_refined
r_symmetry_vdw_other
r_symmetry_hbond_refined
r_symmetry_hbond_other
r_symmetry_metal_ion_refined
r_symmetry_metal_ion_other
r_scangle_it
r_rigid_bond_restr
r_sphericity_free
r_sphericity_bonded
Sample
type III-A Csm-CTR2-dsDNA complex
Sample Components
type III-A Csm-CTR2-dsDNA complex, Csm1 subunit
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentLEICA EM GP
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles75887
Reported Resolution (Å)3.05
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Map-Model Fitting and Refinement
Id1
Refinement Space
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeGATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å**2)60
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
NONE684150