6G8Z

Structure of the pore domain of homomeric mLRRC8A volume-regulated anion channel at 3.66 A resolution

ELECTRON MICROSCOPY

Refinement

RMS Deviations
Key	Refinement Restraint Deviation
f_dihedral_angle_d	10.795
f_angle_d	0.934
f_chiral_restr	0.045
f_bond_d	0.004
f_plane_restr	0.004

Sample
Pore domain of homohexameric mLRRC8A

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	FEI VITROBOT MARK IV
Cryogen Name	ETHANE
Sample Vitrification Details

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	34674
Reported Resolution (Å)	3.66
Resolution Method	FSC 0.143 CUT-OFF
Other Details	The cryo-EM density corresponding to the pore domain was resolved to 3.66 A after performing focused 3D refinement with signal subtraction and C6 symm ...	The cryo-EM density corresponding to the pore domain was resolved to 3.66 A after performing focused 3D refinement with signal subtraction and C6 symmetry.
Refinement Type
Symmetry Type	POINT
Point Symmetry	C6

Map-Model Fitting and Refinement
Id	1
Refinement Space
Refinement Protocol	AB INITIO MODEL
Refinement Target
Overall B Value
Fitting Procedure
Details

Data Acquisition
Detector Type	GATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å**2)	1.1

Imaging Experiment	1
Date of Experiment
Temperature (Kelvin)
Microscope Model	FEI TITAN KRIOS
Minimum Defocus (nm)	500
Maximum Defocus (nm)	3200
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS	2.7
Imaging Mode	BRIGHT FIELD
Specimen Holder Model	FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification	46511
Calibrated Magnification	46511
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	300
Imaging Details

EM Software
Task	Software Package	Version
PARTICLE SELECTION	RELION
IMAGE ACQUISITION	SerialEM
CTF CORRECTION	CTFFIND
MODEL FITTING	Coot
INITIAL EULER ASSIGNMENT	RELION
FINAL EULER ASSIGNMENT	RELION
RECONSTRUCTION	RELION
MODEL REFINEMENT	PHENIX
MODEL REFINEMENT	REFMAC

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION		105867

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.