6ET5

Reaction centre light harvesting complex 1 from Blc. virids


ELECTRON MICROSCOPY

Refinement

RMS Deviations
KeyRefinement Restraint Deviation
r_long_range_B_refined39.469
r_dihedral_angle_2_deg35.143
r_dihedral_angle_3_deg22.411
r_mcangle_it21.459
r_dihedral_angle_4_deg19.042
r_scbond_it16.679
r_mcbond_it13.3
r_dihedral_angle_1_deg8.649
r_angle_refined_deg3.468
r_chiral_restr0.332
RMS Deviations
KeyRefinement Restraint Deviation
r_long_range_B_refined39.469
r_dihedral_angle_2_deg35.143
r_dihedral_angle_3_deg22.411
r_mcangle_it21.459
r_dihedral_angle_4_deg19.042
r_scbond_it16.679
r_mcbond_it13.3
r_dihedral_angle_1_deg8.649
r_angle_refined_deg3.468
r_chiral_restr0.332
r_bond_refined_d0.015
r_gen_planes_refined0.012
r_bond_other_d
r_angle_other_deg
r_gen_planes_other
r_nbd_refined
r_nbd_other
r_nbtor_refined
r_nbtor_other
r_xyhbond_nbd_refined
r_xyhbond_nbd_other
r_metal_ion_refined
r_metal_ion_other
r_symmetry_vdw_refined
r_symmetry_vdw_other
r_symmetry_hbond_refined
r_symmetry_hbond_other
r_symmetry_metal_ion_refined
r_symmetry_metal_ion_other
r_mcbond_other
r_mcangle_other
r_scbond_other
r_scangle_it
r_scangle_other
r_long_range_B_other
r_rigid_bond_restr
r_sphericity_free
r_sphericity_bonded
Sample
Reaction centre-Light harvesting complex 1 (RC-LH1)
Specimen Preparation
Sample Aggregation StateCELL
Vitrification InstrumentLEICA EM GP
Cryogen NameETHANE
Sample Vitrification DetailsBlot for 3.5 seconds. Humidity: 99% Temperature:4C.
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles267726
Reported Resolution (Å)2.87
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1
Refinement SpaceREAL
Refinement ProtocolBACKBONE TRACE
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeGATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å**2)2.25
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification
Calibrated Magnification130000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
PARTICLE SELECTIONRELION2.0
IMAGE ACQUISITIONEPU
CTF CORRECTIONGctf1.06
MODEL FITTINGCoot0.8.7
INITIAL EULER ASSIGNMENTEMAN2
FINAL EULER ASSIGNMENTRELION2.0
CLASSIFICATIONRELION2.0
RECONSTRUCTIONRELION2.0
MODEL REFINEMENTREFMAC5.7
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTIONgctf was used for CTF correction267726Protein was checked using negatively stained EM.