Experiment: 6A8Y

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-1H NOESY	300 uM 15N 13C YR26_SDS	90% H2O/10% D2O	0.3 mM	7	1 atm	298	Bruker DRX 600
2	3D NOESY	300 uM 15N 13C YR26_SDS	90% H2O/10% D2O	0.3 mM	7	1 atm	298	Bruker DRX 600
3	2D 1H-15N HSQC	300 uM 15N 13C YR26_SDS	90% H2O/10% D2O	0.3 mM	7	1 atm	298	Bruker DRX 600
4	2D 1H-13C HSQC	300 uM 15N 13C YR26_SDS	90% H2O/10% D2O	0.3 mM	7	1 atm	298	Bruker DRX 600

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DRX	600

NMR Refinement
Method	Details	Software
na		CYANA

NMR Ensemble Information
Conformer Selection Criteria	lowest energy
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (average)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	chemical shift assignment	Sparky		Goddard
2	structure calculation	CYANA		Guntert, Mumenthaler and Wuthrich
3	collection	TopSpin		Bruker Biospin

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.