NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.8 mM [U-99% 15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.8 mM [U-99% 15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
2 | 2D 1H-15N HSQC | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
3 | 2D 1H-13C HSQC aliphatic | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
4 | 3D CBCA(CO)NH | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
5 | 3D HNCACB | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
12 | 3D HNCO | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
11 | 3D HCCH-TOCSY | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
10 | 3D CCH-TOCSY | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
9 | 3D 1H-13C NOESY | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
8 | 3D 1H-15N NOESY | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.8 mM [U-99% 15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.8 mM [U-99% 15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |
7 | 3D HBHA(CO)NH | 20 mM PBS, 100 mM NaCl, 0.02 mg/mL NaN3, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 1, 0.7 mM [U-13C; U-15N] coiled-coil domain of Drosophila GABAB receptor 2 | 90% H2O/10% D2O | 120 mM | 7.0 | 1 atm | 293 | Agilent DD2 600 600 |