5UYM

70S ribosome bound with cognate ternary complex base-paired to A site codon, closed 30S (Structure III)


ELECTRON MICROSCOPY
Sample
70S ribosome bound with cognate ternary complex base-paired to A site codon, closed 30S (Structure III)
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentGATAN CRYOPLUNGE 3
Cryogen NameETHANE
Sample Vitrification Details2 uL of complex was applied to each grid. After a 10-second incubation, the grids were blotted for 2 to 4 seconds.
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles153597
Reported Resolution (Å)3.2
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement Targetcorrelation coefficient
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeGATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å**2)1
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)500
Maximum Defocus (nm)5000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification60976
Calibrated Magnification60976
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
IMAGE ACQUISITIONSerialEM3
CTF CORRECTIONCTFFIND3
MODEL FITTINGUCSF Chimera1.7
MODEL REFINEMENTRSRef1.1
MODEL REFINEMENTCNS1.3
MODEL REFINEMENTPHENIX1.11
INITIAL EULER ASSIGNMENTFREALIGN9.11
FINAL EULER ASSIGNMENTFREALIGN9.11
CLASSIFICATIONFREALIGN9.11
RECONSTRUCTIONFREALIGN9.11
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTIONCTFFIND3 was used to determine CTF values. FREALIGN applied CTF correction.800367Particles were picked from micrographs using Signature reference-based particle picker.