NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 3D HNCA | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
2 | 3D HN(CO)CA | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
3 | 3D HNCACB | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
4 | 3D HN(COCA)CB | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
5 | 3D HNCO | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
6 | 3D HNCACO | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
7 | 2D 1H-15N HSQC | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
8 | 2D 1H-13C HSQC aliphatic | 10 mM [U-2H] TRIS, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 150 mM sodium chloride, 1 mM sodium azide, 0.02 ug/mL TSP, 0.5 mM EDTA | 100% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 950 |
9 | 2D 1H-13C trosy aromatic | 10 mM [U-2H] TRIS, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 150 mM sodium chloride, 1 mM sodium azide, 0.02 ug/mL TSP, 0.5 mM EDTA | 100% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
10 | 3D 1H-15N NOESY | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 950 |
11 | 3D 1H-13C NOESY aliphatic | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 950 |
12 | 3D HCCH-TOCSY | 10 mM [U-2H] TRIS, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 150 mM sodium chloride, 1 mM sodium azide, 0.02 ug/mL TSP, 0.5 mM EDTA | 100% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
13 | 3D 1H-15N TOCSY | 10 mM TRIS, 150 mM sodium chloride, 0.02 ug/mL TSP, 1 mM sodium azide, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 0.5 mM EDTA | 90% H2O/10% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
14 | 3D 1H-13C TOCSY | 10 mM [U-2H] TRIS, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 150 mM sodium chloride, 1 mM sodium azide, 0.02 ug/mL TSP, 0.5 mM EDTA | 100% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |
15 | 3D 1H-13C NOESY aromatic | 10 mM [U-2H] TRIS, 0.35 mM [U-13C; U-15N] 15th Fibronectin III domain from human fibronectin, 150 mM sodium chloride, 1 mM sodium azide, 0.02 ug/mL TSP, 0.5 mM EDTA | 100% D2O | 161 mM | 6.5 | 1 bar | 298 | Bruker AVANCE III 800 |