5LXY

Structure of the minimal RBM7 - ZCCHC8 Complex

X-RAY DIFFRACTION

Crystallization

Crystalization Experiments
ID	Method	pH	Temperature	Details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.1 M Bis-Tris-Propane 0.2 M NaBr 0.1 M Sodium malonate 20% (w/v) PEG3350

Crystal Properties
Matthews coefficient	Solvent content
2.9	57.3

Crystal Data

Unit Cell
Length ( Å )	Angle ( ˚ )
a = 178.771	α = 90
b = 66.575	β = 126.57
c = 111.912	γ = 90

Symmetry
Space Group	C 1 2 1

Diffraction

Diffraction Experiment
ID #	Crystal ID	Scattering Type	Data Collection Temperature	Detector	Detector Type	Details	Collection Date	Monochromator	Protocol
1	1	x-ray	100	PIXEL	DECTRIS PILATUS 6M		2015-06-26	M	SINGLE WAVELENGTH

Radiation Source
ID #	Source	Type	Wavelength List	Synchrotron Site	Beamline
1	SYNCHROTRON	SLS BEAMLINE X10SA	1.0	SLS	X10SA

Data Collection

Overall
ID #	Resolution (High)	Resolution (Low)	Percent Possible (Observed)	R Merge I (Observed)	CC (Half)	Net I Over Average Sigma (I)	Redundancy	Number Reflections (All)	Number Reflections (Observed)	Observed Criterion Sigma (F)	Observed Criterion Sigma (I)	B (Isotropic) From Wilson Plot
1	2.85	71.79	99.8	0.061	0.995	8.1	3.2		24933

Highest Resolution Shell
ID #	Resolution (High)	Resolution (Low)	Percent Possible (All)	Percent Possible (Observed)	CC (Half)	Mean I Over Sigma (Observed)	Redundancy	Number Unique Reflections (All)
1	2.85	2.92	99.7		0.62

Refinement

Statistics
Diffraction ID	Structure Solution Method	Cross Validation method	Starting model	Resolution (High)	Resolution (Low)	Number Reflections (Observed)	Number Reflections (R-Free)	Percent Reflections (Observed)	R-Work	R-Free	R-Free Selection Details	Mean Isotropic B
X-RAY DIFFRACTION	MOLECULAR REPLACEMENT	THROUGHOUT	5LXR	2.85	71.79	24232	1245	99.78	0.2618	0.3	RANDOM

Temperature Factor Modeling
Anisotropic B[1][1]	Anisotropic B[1][2]	Anisotropic B[1][3]	Anisotropic B[2][2]	Anisotropic B[2][3]	Anisotropic B[3][3]

RMS Deviations
Key	Refinement Restraint Deviation
r_dihedral_angle_2_deg	23.805
r_dihedral_angle_4_deg	13.952
r_dihedral_angle_3_deg	12.573
r_mcangle_it	8.09
r_mcbond_other	5.241
r_mcbond_it	5.24
r_dihedral_angle_1_deg	4.166
r_angle_other_deg	3.767
r_angle_refined_deg	1.147
r_chiral_restr	0.038

RMS Deviations
Key	Refinement Restraint Deviation
r_dihedral_angle_2_deg	23.805
r_dihedral_angle_4_deg	13.952
r_dihedral_angle_3_deg	12.573
r_mcangle_it	8.09
r_mcbond_other	5.241
r_mcbond_it	5.24
r_dihedral_angle_1_deg	4.166
r_angle_other_deg	3.767
r_angle_refined_deg	1.147
r_chiral_restr	0.038
r_bond_refined_d	0.005
r_gen_planes_refined	0.005
r_gen_planes_other	0.004
r_bond_other_d

Non-Hydrogen Atoms Used in Refinement
Non-Hydrogen Atoms	Number
Protein Atoms	5485
Nucleic Acid Atoms
Solvent Atoms	3
Heterogen Atoms	9

Software

Software
Software Name	Purpose
PDB_EXTRACT	data extraction
xia2	data reduction
Aimless	data scaling
REFMAC	refinement
PHASER	phasing

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.