5K0Z

Cryo-EM structure of lactate dehydrogenase (LDH) in inhibitor-bound state


ELECTRON MICROSCOPY
Sample
Lactate dehydrogenase (LDH) in complex with GSK2837808A
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentLEICA EM GP
Cryogen NameETHANE
Sample Vitrification DetailsPlunged into liquid ethane (LEICE EM GP)
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles50865
Reported Resolution (Å)2.8
Resolution MethodFSC 0.143 CUT-OFF
Other DetailsThe primary map in this entry corresponds to the uncorrected reconstruction. A version sharpened using a B-factor of -150 is provided as additional vo ...The primary map in this entry corresponds to the uncorrected reconstruction. A version sharpened using a B-factor of -150 is provided as additional volume data.
Refinement Type
Symmetry TypePOINT
Point SymmetryD2
Map-Model Fitting and Refinement
Id1 (1I0Z)
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeGATAN K2 QUANTUM (4k x 4k)
Electron Dose (electrons/Å**2)60
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)800
Maximum Defocus (nm)2200
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification270000
Calibrated Magnification101000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
CTF CORRECTIONFREALIGN9.10
MODEL FITTINGUCSF Chimera
MODEL REFINEMENTPHENIX
MODEL REFINEMENTCoot
INITIAL EULER ASSIGNMENTFREALIGN9.10
FINAL EULER ASSIGNMENTFREALIGN9.10
RECONSTRUCTIONFREALIGN9.10
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION508402